2017-08-16 10:02:112025-05-24 18:30:39
description
2-iminobutanoate/2-iminopropanoate deaminase, biosynthesis of isoleucine
2-iminobutanoate/2-iminopropanoate deaminase, detoxification of reactive intermediates generated in isoleucine biosynthesis
locus
BSU00480
BSU_00480
geneLength
375
378
function
biosynthesis of isoleucine
deamination of reactive intermediates generated in isoleucine biosynthesis
names
yabJ
yabJ, ridA
outlinks
bsu
BSU00480
BSU_00480
Gene
Coordinates
55,295 → 55,672
55,295 55,672
The protein
Catalyzed reaction/ biological activity
2-iminobutanoate + H2O = 2-oxobutanoate + NH3 [pubmed|22094463]
2-iminopropanoate + H2O = pyruvate + NH3 [pubmed|22094463]
release of ammonia from reactive enamine/imine intermediates of the pyridoxal 5'-phosphate-dependent threonine dehydratase ([[protein|IlvA]]) [pubmed|22094463]
2-iminobutanoate + H2O = 2-oxobutanoate + NH3 [pubmed|22094463]
2-iminopropanoate + H2O = pyruvate + NH3 [pubmed|22094463]
The protein
Protein family
Ref.3 (according to Swiss-Prot) YjgF family
RutC family (single member, according to UniProt)
The protein
Structure
[PDB|1QD9], 1QD9 [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=11484 NCBI] [Pubmed|10557275]
[PDB|1QD9] [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=11484 NCBI] [Pubmed|10557275]
[PDB|5Y6U]
The protein
[SW|Localization]
cytoplasm (according to Swiss-Prot)
cytoplasm (according to UniProt)
Biological materials
Mutant
MGNA-B911 (yabJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1910 NBRP B. subtilis, Japan]
MGNA-B911 (yabJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1910 NBRP B. subtilis, Japan]
BKE00480 ([[gene|yabJ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGTTTTGTGTGGACTGCTT, downstream forward: _UP4_TAATAAGAAAAGTGATTCTG
BKK00480 ([[gene|yabJ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGTTTTGTGTGGACTGCTT, downstream forward: _UP4_TAATAAGAAAAGTGATTCTG
References
The protein
Paralogous protein(s)
[[this]]